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目的 调查海南省不同疟区疟疾流行情况 ,了解其流行趋势 ,为制定防治措施提供依据。方法 应用间接荧光抗体试验 (IFAT)对南桥、毛阳高低疟区进行纵向调查 ,并与镜检法及 PCR技术进行比较研究。结果 南桥、毛阳居民 IFAT抗体阳性率分别为 2 8.85 % (2 96 / 10 2 6 )和 6 .4 7% (6 4 /989) ,低年龄组阳性率为 9.92 % (39/ 393)和 3.98% (14 / 35 2 ) ;原虫阳性率依次为 6 .6 2 % (6 8/ 10 2 6 )和 1.4 2 % (14 / 989) ,PCR与镜检比较 ,阳性符合率为 86 .90 %。结论 南桥居民抗体阳性率和原虫阳性率均明显高于毛阳 ,显示疟疾传播仍较严重 ,毛阳疟疾虽有效控制 ,但仍有低年龄组阳性 ,说明疟疾传播尚未阻断。 PCR可用作流行病学调查和监测。
Objective To investigate the epidemic situation of malaria in different malaria endemic areas in Hainan Province, understand its epidemic trend and provide the basis for making prevention and control measures. Methods The indirect fluorescent antibody test (IFAT) was used to investigate the longitudinal distribution of the malaria strains in south bridge and maoyang, and compared with microscopic examination and PCR. Results The positive rates of IFAT antibody in south bridge and Mao Yang residents were 8.85% (2 96/1062) and 6.47% (64/989) respectively, and those in the lower age group were 9.92% (39/393) And 3.98% (14/35 2) respectively. The positive rates of protozoa were 6.62% (6/8) and 1.4% (14/989) respectively. The positive coincidence rate of PCR and microscopic examination was 86. 90%. Conclusion The antibody positive rate and the positive rate of protozoa in Nanqiao were significantly higher than those in Maoyang, indicating that malaria transmission was still serious. Although malaria control in maoyang was effective, there was still a low positive age group, indicating that malaria transmission has not been blocked yet. PCR can be used for epidemiological investigation and monitoring.