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目的:建立基层适宜的减肥保健食品中添加酚酞的分析方法。方法:采用薄层色谱扫描(TLCS)法。供试品用无水乙醇超声提取,以GF254铝合金硅胶板为固定相,以乙酸乙酯-石油醚(60~90℃)-甲醇(10∶6∶1)为展开剂,采用单波长吸收扫描法,λ=254 nm,狭缝宽8 mm×0.6 mm。结果:酚酞标准曲线线性范围为4.00~20.0μg(r=0.996),平均回收率(n=6)为100.2%。用建立的TLCS法分别对减肥保健食品中共计16个品种20个批次进行酚酞的检测,11个批次检出了酚酞,含量在1.37%~16.51%。结论:所建立的方法经与高效液相色谱-质谱(HPLC-MS)方法比较,可作为减肥保健类产品添加酚酞的定性和定量分析方法。
OBJECTIVE: To establish an appropriate method for the analysis of phenolphthalein added to grassroots weight loss health food. Methods: Thin layer chromatography (TLCS) method was used. The samples were extracted with anhydrous ethanol by ultrasonic extraction. GF254 aluminum alloy silica gel plate was used as the stationary phase. Ethyl acetate-petroleum ether (60-90 ℃) -methanol (10: 06: 1) Scanning method, λ = 254 nm, slit width 8 mm × 0.6 mm. Results: The linear range of phenolphthalein standard curve was 4.00 ~ 20.0μg (r = 0.996). The average recovery (n = 6) was 100.2%. The TLCS method was used to detect phenolphthalein in 20 batches of 16 varieties of weight loss health food products in total, phenolphthalein was detected in 11 batches, and contents ranged from 1.37% to 16.51%. Conclusion: Compared with HPLC-MS method, the established method can be used as a qualitative and quantitative analysis method of adding phenolphthalein as weight loss and health care products.