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OBJECTIVE: To use 2.2.15 cell line to determine the effects of mycophenolate acid (MPA) on hepatitisB virus (HBV) replication and viral protein synthesis in vitro.METHODS: The 2.2.15 cells were treated with different concentration of MPA (1-50 μg/ml) for 12days. HBsAg and HBeAg were detected in the supernatant fluid by ELISA and intracellular HBV DNAwas analyzed quantitatively by slot blot hybridization.RESULTS: MPA could suppress the expression of HBsAg and HBeAg, and the higher concentration ofMPA induced lower expression of HBsAg and HBeAg. The suppression rates of MPA for HBsAg andHBeAg at a concentration of 50 μg/ml were 34.2% and 24.1% respectively. The expression of HBVDNA was only 49% as compared with controls when treated with MPA at a concentration of 50 μg/ml.CONCLUSIONS: Mycophenolate acid can suppress the expression of HBsAg and HBeAg as well as thereplication of HBV DNA in the 2.2.15 cell. The suppressive degree is dose-dependent.
OBJECTIVE: To use 2.2.15 cell lines to determine the effects of mycophenolate acid (MPA) on hepatitis B virus (HBV) replication and viral protein synthesis in vitro. METHODS: The 2.2.15 cells were treated with different concentrations of MPA (1- 50 μg / ml) for 12 days. HBsAg and HBeAg were detected in the supernatant fluid by ELISA and intracellular HBV DNA was analyzed by quantitatively by slot blot hybridization .RESULTS: MPA could suppress the expression of HBsAg and HBeAg, and the higher concentration of MPA induced lower expression of HBsAg and HBeAg. The suppression rates of MPA for HBsAg and HBeAg at a concentration of 50 μg / ml were 34.2% and 24.1% respectively. The expression of HBVDNA was only 49% as compared with controls when treated with MPA at a concentration of 50 μg / ml. CONCLUSIONS: Mycophenolate acid can suppress the expression of HBsAg and HBeAg as a HBV DNA in the 2.2.15 cell. The suppressive degree is dose-dependent.