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目的 探讨各种丙型肝炎病毒 (HCV)抗原在诊断中的作用及其与HCVRNA的关系。方法 利用HCV不同区段抗原抗 -HCV酶联免疫试验 (EIA)和逆转录聚合酶链反应法 (RT PCR) ,对不同临床型HCV患者进行检测。结果 各组患者均以抗 NS3和抗 C阳性率最高。但均有一定比例患者抗 NS4和抗 NS5阳性。与各单个片段抗 HCV阳性率相比 ,抗 HCV总抗体阳性率最高。结论 NS3和C抗原在检测抗 HCV中起很重要作用 ,但NS4和NS5抗原在抗 HCVEIA中的作用也不容忽视。联合应用不同区段HCV抗原将大大提高抗 HCVEIA试剂的灵敏度。
Objective To investigate the role of various hepatitis C virus (HCV) antigens in the diagnosis and its relationship with HCV RNA. Methods Different clinical HCV genotypes were detected by anti-HCV immunoassay (EIA) and reverse transcription polymerase chain reaction (RT PCR). Results The positive rates of anti-NS3 and anti-C in each group were the highest. However, a certain percentage of patients with anti-NS4 and anti-NS5 positive. Compared with the positive rate of anti-HCV of each single fragment, the positive rate of anti-HCV total antibody was the highest. Conclusion NS3 and C antigens play an important role in the detection of anti-HCV. However, the anti-HCV NS4 and NS5 antigens should not be neglected. The combination of different sections of HCV antigen will greatly increase the sensitivity of anti-HCVEIA reagent.