为高效地制备高免疫活性的抗体片段，我们试用了两种新的蛋白酶消化鼠源性单克隆抗体（ｍＡｂ）。纯化的ＩｇＧ１抗肺癌ｍＡｂ经无花果蛋白酶和菠萝蛋白酶消化后，抗体片段的产率分别为最大理论产率的７７．６％和９８．８％。两种酶制备的片段为Ｆ（ａｂ′）２与Ｆａｂ混合物。经细胞结合实验、细胞结合抑制实验及动物模型的肿瘤定位显像证明，两种酶所得片段均具有良好的免疫活性，其中无花果蛋白酶所制片段的免疫活性较高，而菠萝蛋白酶所获酶解片段产率较高 To efficiently prepare high immunocompetent antibody fragments, we tested two novel protease-digested murine monoclonal antibodies (mAbs). After purification of the IgGl anti-lung cancer mAb by protease and bromelain digestion, the yield of antibody fragments was 77.6% and 98.8% of the maximum theoretical yield, respectively. Fragments prepared by both enzymes are a mixture of F (ab ’) 2 and Fab. Cell-binding assay, cell-binding inhibition assay and tumor localization imaging in animal models proved that the fragments obtained by both enzymes had good immunocompetence, of which the protease-made fragment had higher immunocompetence and bromelain obtained enzymolysis Fragment yield higher