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目的探讨脂氧素A4和5-脂氧化酶在子宫内膜异位症患者外周血中的表达及其意义。方法选择2012年2—9月于金华市人民医院因子宫内膜异位症行腹腔镜手术治疗的患者27例为内异组,同期选取因卵巢囊肿行手术治疗的患者30例为对照组。酶联免疫吸附实验测定血清脂氧素A4的水平,实时荧光定量PCR技术检测外周血白细胞5-脂氧化酶mRNA的表达水平,采用LSD-t检验比较2组间脂氧素A4、5-脂氧化酶mRNA的差异,脂氧素A4和5-脂氧化酶mRNA表达水平间的相关性采用直线相关分析。结果内异组患者血清脂氧素A4水平为(330.78±48.62)pg/ml,较对照组(535.93±57.98)pg/ml明显降低(t=14.386,P<0.05);内异组患者白细胞中5-脂氧化酶mRNA表达水平为(1.54±0.54),较对照组(2.32±0.81)明显降低(t=4.232,P<0.05)。2组患者中脂氧素A4和5-脂氧化酶mRNA表达水平呈正相关(P<0.05)。结论脂氧素A4和5-脂氧化酶的表达下降可能参与了子宫内膜异位症的发生。
Objective To investigate the expression of lipoxin A4 and 5-lipoxygenase in peripheral blood of patients with endometriosis and its significance. Methods Twenty-seven patients with endometriosis due to endometriosis in Jinhua People’s Hospital from January to September in 2012 were treated with endometriosis. Thirty patients with ovarian cyst surgically treated during the same period were selected as the control group. The level of serum lipoxin A4 was measured by enzyme-linked immunosorbent assay. The expression of 5-lipoxygenase mRNA in peripheral blood leucocytes was detected by real-time fluorescence quantitative PCR. LSD-t test was used to compare the lipoxygenase A4- Oxidase mRNA differences, lipoxin A4 and 5 - lipoxygenase mRNA expression level correlation between the use of linear correlation analysis. Results The level of serum lipoxin A4 was significantly lower (330.78 ± 48.62) pg / ml than that of the control group (535.93 ± 57.98 pg / ml) (t = 14.386, P <0.05) The mRNA expression of 5-lipoxygenase was (1.54 ± 0.54), which was significantly lower than that of the control group (2.32 ± 0.81) (t = 4.232, P <0.05). Two groups of patients lipoxin A4 and 5-lipoxygenase mRNA expression was positively correlated (P <0.05). Conclusion The decreased expression of lipoxin A4 and 5-lipoxygenase may be involved in the pathogenesis of endometriosis.