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目的 检测乳腺癌患者外周血中CEA mRNA的表达,建立取材于外周血早期诊断乳腺癌微转移的方法。方法采集65例乳腺癌和37例乳腺良性疾病患者的外周血,分离其有核细胞后进行细胞总RNA的抽提,运用巢式RT-PCR技术进行CEA mRNA的检测。结果以巢式RT-PCR终产物出现131 bp带定为阳性。65例乳腺癌患者中,26例CEA mRNA阳性表达,阳性率为40.0％;37例乳腺良性疾病者CEA mRNA均无表达,差异具有显著性(x2=7.41,P<0.001)。结论RT-PCR可以用于临床检测乳腺癌患者外周血中微转移。
Objective To detect the expression of CEA mRNA in peripheral blood of patients with breast cancer and to establish a method for early diagnosis of breast cancer micrometastasis drawn from peripheral blood. Methods Peripheral blood samples from 65 patients with breast cancer and 37 patients with benign breast diseases were collected. Total RNA was extracted from the nucleated cells and nested RT-PCR was used to detect CEA mRNA. The results showed that the 131 bp band of nested RT-PCR products was positive. The CEA mRNA expression was positive in 26 of 65 breast cancer patients, the positive rate was 40.0%. There was no significant difference in CEA mRNA expression in 37 cases of benign breast diseases (x2 = 7.41, P <0.001). Conclusion RT-PCR can be used to detect micrometastasis in peripheral blood of patients with breast cancer.