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IL-12 priming plays an important role in stimulation of CD8+ effector T cells and development of CD8+ memory T (Tm) cells. However,the functional alteration of CD8+ Tm cells developed in the absence of IL-12 priming is elusive. In this study,we investigated the capacity of secondary expansion of CD8+ Tm cells developed from transgenic OT I CD8+ T cells. The latter cells were in vitro and in vivo stimulated by ovalbumin (OVA)-pulsed dendritic cells DCOVA and (IL-12-/-)DCOVA derived from wild-type C57BL/6 and IL-12 gene knockout mice,respectively. We demonstrated that IL-12 priming is important not only in CD8+ T cell clonal expansion,but also in generation of CD8+ Tm cells with the capacity of secondary expansion upon antigen re-encounter. However,IL-12 signaling is not involved in CD8+ Tm cell survival and recall responses. Therefore,this study provides useful information for vaccine design and development.
IL-12 priming plays an important role in stimulation of CD8 + effector T cells and development of CD8 + memory T (Tm) cells. However, the functional alteration of CD8 + Tm cells developed in the absence of IL-12 priming is elusive. , we investigated the capacity of secondary expansion of CD8 + Tm cells developed from transgenic OT I CD8 + T cells. The latter cells were in vitro and in vivo stimulated by ovalbumin (OVA) -pulsed dendritic cells DCOVA and (IL-12 - / -) DCs derived from wild-type C57BL / 6 and IL-12 gene knockout mice, respectively. We demonstrated that IL-12 priming is not only in CD8 + T cell clonal expansion but also in generation of CD8 + Tm cells with the capacity of secondary However, this study provides useful information for vaccine design and development.