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为探讨细胞凋亡在大肠癌形成中的作用,运用DNA原位末端标记法(TUNEL法)对26例大肠癌其中男25冽,女19例,年龄21～68岁,平均39岁,单纯肠炎18例.判定大肠癌细胞凋亡,细胞核呈红色为阳性,阳性程度分为3级,在26例大肠癌标本中均可见到细胞凋亡,18例单纯肠炎仅2例发现有细胞凋亡,细胞凋亡DNA片段经原位末端标记阳性信号位于细胞核内,阳性信号为红色.细胞凋亡主要以弥漫性散在分布,也可呈灶性分布.大肠癌中的细胞凋亡明显高于肠炎(P<0.01).细胞凋亡数与大肠癌分化有关,高分化低于低分化腺癌(P<0.01),Dukes分期明显低于Dukes分期B,C期(P<0.01).结果表明细胞凋亡概念源于形态学改变,用来描述许多组织细胞死亡时所伴随的一系列形态学改变,细胞凋亡最具有的特征性改变是核酸内切酶的激活导致染色质DNA在核小体断裂,形成180～200碱基对的倍数为最小单位的单体或寡聚体片段.本实验采用TUNEL和缺口翻译法显示DNA裂解的原理,对光镜观察证实具有广泛细
In order to explore the role of apoptosis in the formation of colorectal cancer, TUNEL was used to analyze 26 cases of colorectal cancer including 25 males and 19 females, aged from 21 to 68 years, with an average of 39 years old. In 18 cases, the apoptosis of colorectal cancer cells was determined, and the nucleus was red positive. The positive degree was divided into 3 grades. Apoptosis was observed in 26 specimens of colorectal cancer, and only 18 cases were found to have apoptosis in 18 cases of simple enteritis. Apoptotic DNA fragments are located in the nucleus by positive signal in situ. The positive signal is red. Apoptosis is mainly diffusely distributed and can be focally distributed. The apoptosis of colorectal cancer is significantly higher than that of enteritis ( P<0.01). The number of apoptotic cells was related to the differentiation of colorectal carcinoma. The differentiation was significantly lower than that of poorly differentiated adenocarcinoma (P<0.01). The Dukes stage was significantly lower than Dukes stage B and C (P<0.01). The concept of death stems from morphological changes and is used to describe a series of morphological changes that accompany cell death in many tissues. The most characteristic feature of apoptosis is that the activation of endonucleases causes the cleavage of chromatin DNA in the nucleosome. Monomers or oligomers that form a minimum unit of 180-200 base pairs Segment in this experiment by TUNEL and nick translation of DNA cleavage display principle, confirmed by the optical microscope with a wide range of fine