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核酸原位杂交（ ｉｎ ｓｉｔｕ ｈｙｂｉｄｉｚａ－ｔｉｏｎ，ＩＳＨ）技术，自１９６９年建立以来，其技术和方法得到了不断的改进与完善，目前已被广泛地应用于生命科学各个领域。原位杂交是分子生物学与组织化学的成功结合，其原理是：利用放射性或非放射性标记的、含有互补
In situ hybidiza-tion (ISH) technology has been continuously improved and perfected since it was established in 1969, and has been widely used in all fields of life science at present. In situ hybridization is the successful combination of molecular biology and histochemistry, the principle is: the use of radioactive or non-radioactive labeling, containing complementary