论文部分内容阅读
目的考察小檗碱配伍水苏糖对自发性2型糖尿病KKAy小鼠糖脂代谢及肠道菌群的影响。方法将2型糖尿病KKAy雌性小鼠随机分为模型组(水0.1 m L·10 g~(-1))、小檗碱组(小檗碱100 mg·kg~(-1)·d~(-1))、水苏糖组(水苏糖200 mg·kg~(-1)·d~(-1))及联合组(小檗碱100 mg·kg~(-1)·d~(-1)+水苏糖200mg·kg~(-1)·d~(-1)),每组9只;另随机选取10只C57BL/6J雌性小鼠作为正常对照组(水0.1 m L·10 g~(-1))。5组小鼠每日灌胃给药一次,连续7周。检测小鼠血糖、血脂、口服葡萄糖耐量及胰岛素敏感性的变化,并用基于16SrRNA的实时荧光定量聚合酶链式反应技术分析小鼠肠道中乳酸杆菌及双歧杆菌丰度的变化。结果与模型组相比,联合组可显著降低KKAy小鼠的空腹血糖、血清总胆固醇水平(P<0.05),改善其口服葡萄糖耐量异常(P<0.05)及胰岛素抵抗(P<0.01),还可显著上调小鼠肠道益生菌乳酸杆菌和双歧杆菌丰度(P<0.01),且其作用明显优于小檗碱组及水苏糖组。结论小檗碱配伍水苏糖能明显改善糖尿病KKAy小鼠的糖脂代谢紊乱,且作用效果相比于单一成分更具优越性,其机制可能与促进肠道益生菌乳酸杆菌及双歧杆菌的增殖有关。
Objective To investigate the effects of berberine and stachyose on glucose and lipid metabolism and intestinal microflora in KKAy mice with spontaneous type 2 diabetes mellitus. Methods KKAy female mice with type 2 diabetes mellitus were randomly divided into model group (water 0.1 m L · 10 g -1), berberine group (berberine 100 mg · kg -1 · d -1 (100 mg · kg -1 · d -1), stachyose group (stained with stachyose 200 mg · kg -1 · d -1) and combination group (berberine 100 mg · kg -1 · d -1) 1) and stachyose 200 mg · kg -1 · d -1, with 9 mice in each group. Ten female C57BL / 6J mice were randomly selected as the normal control group (water 0.1 m L · 10 g -1). Five groups of mice were orally administered once daily for 7 weeks. The changes of blood glucose, blood lipid, oral glucose tolerance and insulin sensitivity in mice were detected. The changes of the abundance of Lactobacillus and Bifidobacterium in intestinal tract of mice were analyzed by 16SrRNA-based real-time fluorescent quantitative polymerase chain reaction. Results Compared with the model group, the combination group significantly reduced fasting blood glucose and serum total cholesterol (P <0.05), and improved oral glucose tolerance (P <0.05) and insulin resistance (P <0.01) Could significantly increase the abundance of Lactobacillus and Bifidobacterium in mice intestinal probiotics (P <0.01), and its effect was significantly better than that of berberine group and stachyose group. Conclusion Berberine combined with stachyose can obviously improve the disorder of glycolipid metabolism in diabetic KKAy mice, and the effect is more superior to that of single component. The mechanism may be related to the promotion of intestinal probiotics Lactobacillus and Bifidobacterium Proliferation related.