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目的:系统比较野生甘草无性繁殖的甘草根和人工甘草种子有性繁殖的甘草根中药用成分的积累速度差异情况,为进一步研究影响甘草药用成分积累的基因机制奠定理论基础。方法:本研究基于HPLC和UV等分析方法,比较野生甘草和人工甘草在甘草酸、甘草苷和甘草总黄酮以及其他类成分的含量差异。基于HPLC指纹图谱技术,全面分析不同繁殖模式下甘草根的化学信息和内在质量的差异情况。结果:除甘草素和异甘草素外,野生甘草不定根中其他三种药用成分和总黄酮的含量均高于人工甘草实生根,经SPSS分析,除异甘草素外,其他成分在两组之间均存在显著差异;根据HPLC指纹图谱,两种甘草药用成分之间差异显著,相似度为0.647。结论:野生无性繁殖的甘草根和人工有性繁殖的甘草根中主要药用成分含量之间存在显著差异,野生甘草具有快速积累药用成分的功能。本研究结果对于今后人工调控生产优质甘草药材,实现甘草资源的可持续利用具有重要的理论和实践意义。
OBJECTIVE: To systematically compare the differences in the accumulation rate of medicinal components of licorice roots between licorice root of wild licorice and licorice root of artificial licorice root in order to further study the gene mechanism that affects the accumulation of medicinal components of licorice root. Methods: The HPLC-UV and other analytical methods were used to compare the contents of glycyrrhizin, glycyrrhizin and licorice flavonoids and other components of wild licorice and artificial licorice. Based on HPLC fingerprinting, the differences of chemical information and intrinsic quality of licorice root under different reproduction modes were comprehensively analyzed. Results: The contents of three other medicinal components and total flavonoids in adventitious roots of wild licorice were higher than those of artificial licorice root except for the content of liquiritigenin and isoliquiritigenin. According to SPSS analysis, except the isoliquiritigenin, the other components in the two groups There was a significant difference between them. According to HPLC fingerprinting, there was significant difference between the two medicinal compositions of licorice, the similarity was 0.647. CONCLUSION: There is a significant difference in the content of main medicinal components among wild vegetative licorice root and artificially propagated licorice root. Wild licorice has the function of quickly accumulating medicinal components. The results of this study have important theoretical and practical significance for the future production of high-quality licorice by artificial control, and the sustainable utilization of licorice resources.