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本文报告了茯苓素对人急性早幼粒白血病细胞系HL-60增殖的抑制作用.经25～100μg/ml茯苓素处理1天,HL-60细胞DNA合成能力明显减弱.药物作用2天后,细胞计数明显少于对照组.这种效应随药物浓度的加大及作用时间的延长而增强.半数抑制浓度为58.4μg/ml.抑制作用为不可逆性.茯苓素处理4天后,HL-60细胞自发形成集落的能力明显减弱.细胞周期分析结果,加药后G_0/G_1期细胞明显增多,S和G_2+M期细胞减少.
In this paper, the inhibitory effect of Poria on the proliferation of human acute promyelocytic leukemia cell line HL-60 was reported. The DNA synthesis ability of HL-60 cells was significantly weakened after treated with 25 ~ 100μg / ml Porcin for 1 day. After 2 days of drug treatment, And the count was significantly less than the control group.This effect increased with the increase of drug concentration and the extension of time.The half inhibitory concentration was 58.4μg / ml.The inhibitory effect was irreversible.After 4 days of Poria treatment, HL-60cells spontaneously The ability to form colonies was significantly weakened.Cell cycle analysis showed that the number of G 0 / G 1 phase cells increased significantly and the number of S and G 2 + M phase cells decreased after dosing.