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目的:采用高效液相法测定贯叶连翘中的金丝桃素、芦丁和槲皮素的含量方法。方法:色谱柱为SymmtryC18(4.6mm×250mm,5μm),检测波长分别为588nm,359nm,流动相分别为甲醇-乙腈-1.0%磷酸二氢钠溶液(340∶12∶7)和甲醇-水(40∶60)。结果:线性范围分别在0.02704~0.1352μg和0.264~1.32μg,0.052~0.260μg内良好,回收率分别为98.39%(n=3)、98.56%(n=3)和98.85%(n=3)。RSD分别为0.78%和1.01%和1.02%。结论:该法简便,精密度高,结果准确可靠。
Objective: To determine the contents of hypericin, rutin and quercetin in Hypericum perforatum by HPLC. METHODS: The chromatographic column was a Symmetry C18 (4.6 mm×250 mm, 5 μm). The detection wavelengths were 588 nm and 359 nm, respectively. The mobile phases were methanol-acetonitrile-1.0% sodium dihydrogen phosphate solution (340:12:7) and methanol-water ( 40:60). Results: The linear ranges were good in 0.02704~0.1352μg and 0.264~1.32μg, respectively, and 0.052~0.260μg. The recoveries were 98.39% (n=3), 98.56% (n=3) and 98.85% (n=3) respectively. . The RSDs were 0.78% and 1.01% and 1.02%, respectively. Conclusion: The method is simple, high precision, accurate and reliable results.