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对小鼠体外肝细胞进行了内毒素（ｌｉｐｏｐｏｌｙｓａｃｃｈａｒｉｄｅ，ＬＰＳ）直接毒性作用的研究。发现ＬＰＳ对肝细胞有明显损害作用，表现为肝细胞变性、坏死，存活率降低、培养上清谷草转氨酶（ＡＳＴ）增高，上述改变程度与ＬＰＳ加入量成正比。肝细胞单独培养组与肝细胞－肝非实质细胞混合培养组相比，各指标差异无显著性（Ｐ＞０．０５）。然而，经Ｄ－氨基半乳糖及ＬＰＳ体内预处理，导致肝损伤后分离肝非实质细胞，以其取代正常的肝非实质细胞，此时肝非实质细胞的存在不仅加重ＬＰＳ所致的肝细胞损害，且对正常肝细胞也有轻度损伤作用，提示被激活的肝非实质细胞可加重ＬＰＳ所致的肝细胞损害。
The direct hepatotoxicity of lipopolysaccharide (LPS) in mice was studied. It was found that LPS had a significant damage on hepatocytes, manifested as degeneration and necrosis of hepatocytes, decreased survival rate, increased aspartate aminotransferase (AST) in culture supernatant, and the degree of change was directly proportional to the amount of LPS added. There was no significant difference in each index between hepatocytes cultured alone and hepatocytes - hepatocyte non - parenchymal cells mixed culture group (P> 0.05). However, pretreatment with D-galactosamine and LPS in vivo resulted in the isolation of hepatic parenchymal cells after liver injury and their replacement for normal non-parenchymal cells, in which case the presence of hepatic parenchymal cells not only aggravated the LPS-induced hepatocytes Damage, and mild damage to normal liver cells, suggesting that activated liver non-parenchymal cells may increase liver damage caused by LPS.