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目的研究低频电磁场(LF-EMF)刺激左侧星状神经节(LSG)对其功能和神经活性的影响及其机制。方法16只健康成年家犬随机分为LF-EMF组(n=8)和对照组(n=8),电磁场刺激仪刺激探头固定至家犬左侧第二、三肋与脊柱连接的皮肤表面定位于LSG,参数设置:刺激频率:1 Hz,刺激模式:开启8s,暂停5s,刺激时间:2h。LF-EMF组接受电磁场刺激,对照组接受假刺激。在基础状态和2hLF-EMF后分别测定LSG神经活性和高频电刺激(HFS:频率20Hz,脉宽0.1ms)LSG时的血压改变,实时定量聚合酶链反应检测LSG中2型小电导钙激活钾通道分子(SK2)、早期原癌基因(c-fos)和神经生长因子(NGF)mRNA表达水平。结果基础状态时,HFS刺激LSG引起的血压升高作用和神经活性在对照组和LL-EMF组中未见统计学差异;但2hLF-EMF刺激后,相比于对照组,LF-EMF组中HFS刺激LSG引起的血压升高作用明显被抑制(32%±5%vs 43%±5%,P<0.05),LSG神经活性显著降低[频率:(17±5)次/分vs(30±6)次/分;振幅:(0.08±0.02)mV vs(0.16±0.02)mV,P<0.05)],同时LSG中SK2mRNA表达水平明显增高(1.64±0.12vs 1.00±0.09,P<0.05),c-fos和NGF mRNA表达水平明显降低(c-fos:0.54±0.07vs 1.0±0.1,P<0.05;NGF:0.67±0.08vs 1.0±0.16,P<0.05)。结论 LF-EMF可通过调控神经相关蛋白表达水平抑制LSG功能及活性。
Objective To investigate the effects and mechanisms of left stellate ganglion (LSG) on the function and neuroactivity stimulated by LF-EMF. Methods Sixteen healthy adult dogs were randomly divided into LF-EMF group (n = 8) and control group (n = 8). Electromagnetic stimulator was used to fix the probe to the second, third rib and spine connected skin surface Positioning in LSG, parameter setting: stimulation frequency: 1 Hz, stimulation mode: open 8s, pause 5s, stimulation time: 2h. The LF-EMF group received electromagnetic stimulation while the control group received sham stimulation. LSG neural activity and high-frequency electrical stimulation (HFS: frequency 20Hz, pulse width 0.1ms) LSG blood pressure changes were measured after basal state and 2hLF-EMF, real-time quantitative polymerase chain reaction was used to detect type 2 small conductance calcium activation Potassium channel (SK2), early proto-oncogene (c-fos) and nerve growth factor (NGF) mRNA expression levels. Results In the basal state, there was no significant difference between the control group and the LL-EMF group in the effects of HFS stimulation on blood pressure and neurological activity induced by LSG. However, after 2-hLF-EMF stimulation, compared with the control group, The effect of HFS on the increase of blood pressure induced by LSG was significantly inhibited (32 ± 5% vs 43% ± 5%, P <0.05) and the neurological activity of LSG was significantly reduced [frequency: (17 ± 5) / min vs (0.06 ± 0.02) mV vs (0.16 ± 0.02) mV, P <0.05). Meanwhile, the expression of SK2 mRNA in LSG was significantly higher (1.64 ± 0.12 vs 1.00 ± 0.09, P <0.05) c-fos and NGF mRNA expression levels were significantly lower (c-fos: 0.54 ± 0.07 vs 1.0 ± 0.1, P <0.05; NGF: 0.67 ± 0.08 vs 1.0 ± 0.16, P <0.05). Conclusion LF-EMF can inhibit the function and activity of LSG by regulating the expression of neural related proteins.