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目的:建立同时测定野三七中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1及人参皂苷Rd四种三萜皂苷成分含量的高效液相色谱方法。方法:采用Agilent Zorbax SB-C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈(A)-水(B),梯度洗脱(0～5 min,15%A→20%A,5～30 min,20%A→23%A,30～50 min,23%A→40%A,50～55 min,40%A→40%A),流速1 ml·min-1,检测波长203 nm,柱温30℃。结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1及人参皂苷Rd分别在0.122～2.440μg、0.524～10.480μg、0.454～9.080μg及0.370～7.400μg范围内呈良好线性关系(r>0.999 7);野三七药材中4种三萜皂苷成分的平均回收率(n=6)分别为98.83%、99.28%、100.82%、99.06%;RSD分别为1.34%、1.05%、1.14%、1.53%。结论:该方法简便、准确,分离效果好,首次用于野三七药材的质量评价。
OBJECTIVE: To establish a high performance liquid chromatography (HPLC) method for the simultaneous determination of three triterpenoid saponins in Notoginseng saponins R1, Ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd. METHODS: The mobile phase consisted of acetonitrile (A) -water (B), gradient elution (0-5 min, 15% A → 20% A, 5 to 30 min, 20% A to 23% A, 30 to 50 min, 23% A to 40% A, 50 to 55 min, 40% A to 40% 203 nm, column temperature 30 ℃. Results: The concentrations of notoginsenoside R1, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rd in the range of 0.122 ~ 2.440μg, 0.524 ~ 10.480μg, 0.454 ~ 9.080μg and 0.370 ~ 7.400μg were linear (r> 0.999 7 ). The average recoveries of four triterpene saponins from Yehsanqi were 98.83%, 99.28%, 100.82% and 99.06%, respectively; the RSDs were 1.34%, 1.05%, 1.14% and 1.53% . Conclusion: The method is simple and accurate, and has good separation effect. It is the first time that it is used in the quality evaluation of wild Sanqi medicines.