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该文旨在探索前肽缺失的von Willebrand因子(vWF-ΔPro)对蛋白质剪接的L303E/F309S突变体凝血第八因子(FVIII)分泌的影响。将vWF-ΔPro基因与蛋白内含子融合的FVIII重链和轻链基因共转HEK293细胞。结果显示,转vWF-ΔPro细胞的剪接蛋白FVIII分泌量和活性分别为(196±27)ng/mL和(1.39±0.31)IU/mL,明显高于对照细胞的(116±24)ng/mL和(0.91±0.18)IU/mL。表明vWF-ΔPro可提高剪接的L303E/F309S突变体FVIII蛋白分泌量和活性。
This paper aims to explore the effect of von Willebrand factor (vWF-ΔPro) of pro-peptide deletion on the secretion of coagulation factor VIII (FVIII) from the protein spliced L303E / F309S mutant. The FVIII heavy and light chain genes fused vWF-ΔPro gene to the intein were co-transfected into HEK293 cells. The results showed that the spike protein FVIII secretion and activity of vWF-ΔPro cells were (196 ± 27) ng / mL and (1.39 ± 0.31) IU / mL, respectively, which were significantly higher than those of control cells (116 ± 24) ng / mL And (0.91 ± 0.18) IU / mL. It was shown that vWF-ΔPro increased the amount and activity of spliced L303E / F309S mutant FVIII protein.