论文部分内容阅读
采用厚朴酚键合硅胶固定相(MSP),建立了一种同时测定复方氨酚甲麻口服液中愈创木酚磺酸钾、对乙酰氨基苯酚和咖啡因的反相高效液相色谱法。流动相为甲醇-水(40:60,V/V),流速为0.8 mL/min,检测波长为270 nm。研究结果表明,在该色谱条件下,3种化合物能够实现基线分离,其峰面积与浓度呈良好线性关系,愈创木酚磺酸钾、对乙酰氨基苯酚和咖啡因的线性范围分别为5.50～27.50μg/mL、10.40～52.00μg/mL和1.14～22.80μg/mL,相关系数分别为0.9996、0.9999和0.9999。愈创木酚磺酸钾的平均回收率为92.4%～112.9%(RSD<4.6%),对乙酰氨基苯酚的平均回收率为101.5%～104.9%(RSD<0.90%),咖啡因的平均回收率为73.3%～87.8%(RSD<0.72%)。本方法能够满足实际样品的快速常规分析要求。
A new method for the simultaneous determination of potassium guaiacol sulfonate and acetaminophen and caffeine in Compound Aminophenol Kamo Oral Liquid was established by using magnolol phenol bonded silica gel stationary phase (MSP). The reverse phase high performance liquid chromatography . The mobile phase was methanol-water (40:60, V / V), the flow rate was 0.8 mL / min and the detection wavelength was 270 nm. The results show that under the chromatographic conditions, the three compounds can achieve baseline separation, the peak area and concentration showed a good linear relationship, the linear range of potassium guaiacol sulfonate, acetaminophen and caffeine were 5.50 ~ 27.50μg / mL, 10.40 ~ 52.00μg / mL and 1.14 ~ 22.80μg / mL, the correlation coefficients were 0.9996, 0.999 and 0.9999, respectively. The average recovery of potassium guaiacol sulfonate was 92.4% ~ 112.9% (RSD <4.6%), and the average recovery of paracetamol was 101.5% -104.9% (RSD <0.90%). The average recoveries of caffeine Rates ranged from 73.3% to 87.8% (RSD <0.72%). This method meets the fast routine analysis requirements of actual samples.