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采用高效液相色谱串联紫外检测器法(HPLC-UV)和紫外分光光度法(UV)分别对食用油中特丁基对苯二酚(TBHQ)及其氧化产物特丁基对苯醌(TQ)进行测定,并对样品中TBHQ和TQ含量随时间变化规律进行了分析。测定结果显示HPLC-UV法测定TBHQ和TQ线性范围分别为(1.0~100.0)mg/kg(R_(TBHQ)~2=0.9984)、(0.2~35.0)mg/kg(R_(TQ)~20.9986),UV法测定TBHQ和TQ线性范围分别为(1.0~100.0)mg/kg(R2TBHQ=0.9998),(0.5~40.0)mg/kg(R_(TQ)~2=0.9997),2种方法的加标回收率落在89.5%~105.8%之间,相对标准偏差小于8.1%。通过对使用过程中食用油中的TBHQ与TQ含量变化进行了追踪分析,对研究食用油使用次数及使用时间具有重要意义。
High performance liquid chromatography with tandem UV detector (HPLC-UV) and ultraviolet spectrophotometry (UV) were used to detect the content of TBHQ and its oxidation products, such as TQ ) Were measured, and the sample TBHQ and TQ content with time changes were analyzed. The results showed that the linear ranges of TBHQ and TQ were (1.0 ~ 100.0) mg / kg (R_ (TBHQ) ~ 2 = 0.9984) and (0.2 ~ 35.0) mg / The linear ranges of TBHQ and TQ were (1.0 ~ 100.0) mg / kg (R2TBHQ = 0.9998) and (0.5 ~ 40.0) mg / kg (R TQ ~ 2 = 0.9997) The recoveries ranged from 89.5% to 105.8% with relative standard deviations less than 8.1%. Through the use of edible oil in the TBHQ and TQ content were tracked changes in the study of the number of edible oil use and the use of time is of great significance.