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根据编码人IgG1Fc(Fcγ1 )的基因序列 ,设计合成 1对与之相匹配的引物。采用RT PCR法 ,从正常人白细胞mRNA中扩增获得预期目的基因Fcγ1 70 0bp基因片段 ,成功构建PUC1 8 Fcγ1 70 0 重组克隆载体 ,酶切、酶谱分析与预期结果相符 ;DNA测序结果与GenBank报道一致。
According to the gene sequence encoding human IgG1Fc (Fcγ1), we designed and synthesized a pair of matched primers. The RT-PCR method was used to amplify the target gene Fcγ1 70 0bp from normal human leukocyte mRNA, and the recombinant plasmid pUC1 8 Fcγ1 70 0 was constructed successfully. Enzyme digestion and enzyme analysis were consistent with the expected results. The DNA sequencing results were identical to those of GenBank Reported consistent.