论文部分内容阅读
目的评估CT导引经皮纳米刀消融猪肝组织的可行性及安全性。方法 10头成年巴马小型猪随机分为实验组(n=7)和对照组(n=3),全身麻醉下将Nano Knife纳米刀双针电极经CT导引穿刺至肝脏肝门区及肝脏近胆囊处,依标准参数进行消融。术后2 h、2 d、7 d、14 d取材作生化、病理学检查。结果所有实验过程均顺利,未出现出血、感染、气胸、死亡等严重并发症,所有实验猪均存活至实验终点。生化指标检测提示肝功能及心肌酶谱指标出现一过性升高,消融后14 d逐渐恢复正常;病理学检查提示消融后2 d内消融区细胞完全死亡,血管内皮轻度损伤,2 d后产生再次内皮化,14 d后平滑肌细胞重新长入。结论纳米刀消融邻近肝门大血管、胆管、胆囊的猪肝脏组织是安全可行的,为纳米刀技术临床应用提供了大动物实验依据。
Objective To evaluate the feasibility and safety of CT guided percutaneous nano-knife ablation of porcine liver tissue. Methods Ten adult Bama miniature pigs were randomly divided into experimental group (n = 7) and control group (n = 3). The Nano Knife needle was double-needle guided by CT to the hepatic hilar region and liver under general anesthesia Near the gallbladder Department, according to the standard parameters of ablation. At 2 h, 2 d, 7 d and 14 d after operation, biochemical and pathological examination were performed. Results All the experiments were well and no serious complications such as hemorrhage, infection, pneumothorax and death were found. All experimental pigs survived to the end of the experiment. Biochemical tests showed that the indexes of liver function and myocardial enzymes increased transiently and returned to normal 14 days after ablation. The pathological examination showed that the cells in the ablation zone died completely within 2 days after ablation and the vascular endothelial cells were mildly damaged. After 2 days Endothelialization again, 14 days after smooth muscle cells to re-enter. Conclusion Nano-knife ablation of the liver adjacent to the large portal blood vessels, bile duct, gallbladder liver tissue is safe and feasible, for the clinical application of nano-knife technology provides a large animal experimental basis.