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目的探讨抗凋亡基因ｂｃｌ－２蛋白与Ｆａｓ抗原在病毒性肝炎肝组织中表达和分布的关系。方法采用免疫组织化学双标记技术，以ｂｃｌ－２癌蛋白单克隆抗体、抗－ＨＢｓ单克隆抗体及抗－Ｆａｓ多克隆抗体，检测８７例急、慢性肝炎患者肝组织中ｂｃｌ－２、Ｆａｓ及ＨＢｓＡｇ的表达和分布。结果６２例（７１．３％）肝细胞中检出ｂｃｌ－２癌蛋白，阳性物质主要表达于肝细胞胞浆内，少数位于核膜。在急性轻型肝炎，阳性细胞于小叶内弥散分布；在慢性肝炎则多聚集在碎屑样坏死周围。Ｆａｓ抗原的检出率为７５．９％（６６／８７），其阳性细胞的分布类同ｂｃｌ－２阳性肝细胞，但不如后者广泛。ｂｃｌ－２／Ｆａｓ双标记染色显示较多的肝细胞有两种抗原的双表达。结论乙型病毒性肝炎肝组织中ｂｃｌ－２的表达和分布与Ｆａｓ抗原密切相关，ｂｃｌ－２原癌基因可能具有抑制Ｆａｓ介导肝细胞凋亡的作用。
Objective To investigate the relationship between the expression of anti-apoptotic gene bcl-2 protein and Fas antigen in liver tissues of viral hepatitis. Methods The expression of bcl-2, Fas, and Fas in liver tissues of 87 patients with acute and chronic hepatitis were detected by immunohistochemical double-labeling technique with monoclonal antibody against bcl-2 oncoprotein, anti-HBs monoclonal antibody and anti-Fas polyclonal antibody HBsAg expression and distribution. Results The bcl-2 oncoprotein was detected in 62 (71.3%) hepatocytes. The positive cells were mainly expressed in the cytoplasm of hepatocytes, with a few in the nuclear membrane. In acute hepatitis, positive cells diffuse within the leaflets; in chronic hepatitis, they accumulate more around debris-like necrosis. The detection rate of Fas antigen was 75.9% (66/87). The distribution of Fas antigen was similar to that of bcl-2-positive hepatocytes but not as broad as the latter. Bcl-2 / Fas double-labeled staining showed more hepatocytes double expression of two antigens. Conclusion The expression and distribution of bcl-2 in liver tissue of patients with viral hepatitis B is closely related to Fas antigen, and the bcl-2 proto-oncogene may have the effect of inhibiting Fas-mediated hepatocyte apoptosis.