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目的了解调查年份不同种类海、水产品中分离的霍乱弧菌的血清学分型、噬菌体-生物分型及毒力因子携带情况,分析海水产品污染状况与疫情的关系,为疫情预测和制定防治对策提供参考。方法应用血清学分型及噬菌体-生物分型方法分析所分离菌株的生物表型,应用 PCR 方法检测毒力基因的携带情况。结果检测的64株菌株中0139型菌株占48.44%(31/64),01群小川型占31.25%(20/64),01群稻叶型占20.31%(13/64);O1群噬菌体-生物分型显示均为非流行株;对61株菌毒力基因检测结果显示:O139霍乱弧菌毒力基因的检出率(83.33%)远高于 O1群小川型霍乱弧菌(15.38%)和 O1群稻叶型霍乱弧菌(11.1l%)。结论 O139型霍乱弧菌主要存在于甲鱼、甲鱼养殖水及甲壳类海水产品中,而且多为产毒株,这些海、水产品是预防和控制霍乱疫情的重要环节。
Objective To understand the serotyping, phage-biodistribution and virulence factors of Vibrio cholerae isolated from different kinds of sea and aquatic products during the year of investigation, and to analyze the relationship between the pollution status of seawater products and epidemic situation, to forecast the epidemic situation and to make prevention and control measures for reference. Methods The biological phenotypes of the isolates were analyzed by serological typing and phage-typing methods. The virulence genes were detected by PCR. Results Among the 64 strains tested, 0139 strains accounted for 48.44% (31/64), 01 Ogawa type (31.25%) and 01 (31.3%) rice leaf group. O1 phage- The results of 61 strains of virulence genes showed that the detection rate of V. cholerae O139 virulence gene (83.33%) was much higher than that of O1 group (15.38%) And O1 group of Vibrio cholerae (11.1%). Conclusion Vibrio cholerae O139 is mainly found in turtle, turtle farming water and crustacean marine products, and most of them are producing strains. These marine and aquatic products are important links in prevention and control of cholera epidemic.