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为构建棉铃虫精氨酸激酶基因(AK)的原核表达系统及确定2种不同品系的棉铃虫体内AK的表达量差异,以pET-28a质粒为表达载体,将AK基因重组转化至大肠杆菌BL21中进行原核表达。采用SDS-PAGE电泳及实时荧光PCR定量分析。结果表明:经IPTG诱导AK基因在大肠杆菌BL21得到表达,表达的融合蛋白其分子量与预测结果一致;石河子地区田间品系棉铃虫体内AK基因的表达量远远大于敏感品系棉铃虫体内AK基因的表达量为77.36%,表明石河子田间品系棉铃虫的生命代谢活动的能力要强于敏感品系。
To construct a prokaryotic expression system for the arginine kinase gene (AK) of Helicoverpa armigera and to determine the AK expression in two different strains of cotton bollworm, the AKT gene was transformed into E.coli BL21 with pET-28a as the expression vector In prokaryotic expression. SDS-PAGE electrophoresis and real-time fluorescence quantitative PCR analysis. The results showed that AKT gene was induced by IPTG in E. coli BL21, and the molecular weight of the expressed fusion protein was consistent with the predicted result. The expression of AK gene in cotton bollworm in Shihezi was much higher than that in the susceptible strain The amount of 77.36%, indicating that Shihezi field cotton bollworm life metabolic activity ability is stronger than the sensitive strains.