重组人可溶性CD5 9(rhsCD5 9)。将rhsCD5 9的基因克隆于表达载体PinPointXa 3的多克隆位点 ,构建成生物素化sCD5 9融合蛋白表达载体pPinPointXa sCD5 9,将此表达载体转化大肠杆菌JM10 9,IPTG诱导表达后 ,收集裂解菌体 ,取上清过已处理好的SoftLink亲和素树脂柱 ,分段收集融合蛋白 ,以Xa切割后再过SoftLink柱获得rhsCD5 9,初步鉴定rhsCD5 9的生物活性。rhsCD5 9的基因能在大肠杆菌中稳定表达并具有生物活性 Recombinant human soluble CD5 9 (rhsCD5 9). The rhsCD5 9 gene was cloned into the multi-cloning site of the expression vector PinPointXa 3 to construct a biotinylated sCD5 9 fusion protein expression vector pPinPointXa sCD5 9. The expression vector was transformed into E. coli JM109 and induced by IPTG. The supernatant was treated with SoftLink avidin resin column, the fusion protein fragments were collected, cut with Xa after SoftLink column obtained rhsCD5 9 preliminary identification of rhsCD5 9 biological activity. The gene of rhsCD5 9 is stably expressed in E. coli and has biological activity