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BACKGROUND 99m Tc-3PRGD2.SPECT/CT is a commonly used examination method in nuclear medicine. However, patients receiving99m Tc-3PRGD2.SPECT/CT have insufficient knowledge of this method and worry about the examination results.AIM To investigate the effect of teach-back health education combined with structured psychological nursing on adverse emotion and cooperation in patients undergoing99m Tc-3PRGD2.SPECT/CT examination.METHODS Ninety patients undergoing99m Tc-3PRGD2.SPECT/CT examinations were divided into a study group and a control group using a simple random number table, and 45 cases were allocated to each group. Routine nursing was provided to the control group, and teach-back health education combined with structured psychological nursing was provided to the study group on the basis of the control group. Heart rate, diastolic blood pressure, systolic blood pressure, self-rating depression scale（SDS）, and self-rating anxiety scale（SAS） were assessed before and after the intervention, and examination cooperation and intervention satisfaction were assessed in the two groups before, during, and after the examination.RESULTS Before the examination, heart rate, diastolic blood pressure, and systolic blood pressure in the study group were not significantly different from the values of the control group（P ＞ 0.05）. The results of the study group before and after the examination were lower than those in the control group（P ＜ 0.05）. Before the intervention, SDS and SAS scores in the study group were not significantly different from those in the control group（P ＞ 0.05）. After the intervention, SDS and SAS scores in the study group were lower than those in the control group（P ＜ 0.05）. The degree of cooperation was higher in the study group than in the control group（P ＜ 0.05）. The satisfaction rate with the intervention was higher in the study group than in the control group（P ＜ 0.05）.CONCLUSION Teach-back health education combined with structured psychological nursing can help maintain the stability of blood pressure and heart rate, relieve negative emotions, and improve the satisfaction and cooperation of patients undergoing 99m Tc-3PRGD2.SPECT/CT examinations.