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目的:研究升麻Cimicifuga foetida根茎的化学成分和所得化合物的抑制破骨细胞活性。方法:采用正、反相硅胶等色谱方法进行化学成分的分离纯化,根据谱学数据结合理化性质鉴定化合物结构。并进行了抑制破骨细胞活性研究。结果:从升麻根茎的石油醚和醋酸乙酯提取物中共分得13个环菠萝蜜烷三萜成分,其结构分别鉴定为25-O-乙酰基-升麻醇(1),升麻醇(2),升麻酮醇(3),26-去羟基阿科特素(4),类叶升麻苷A(5),23-表-26-去羟基阿科特素(6),阿科特素(7),12β-羟基升麻醇(8),升麻苷E(9),(23R,24S)25-O-乙酰基-升麻醇-3-O-β-D吡喃木糖苷(10),(23R,24S)升麻醇-3-O-β-D-吡喃木糖苷(11),升麻苷H-1(12)和升麻苷H-2(13)。结论:化合物5为首次从该属植物中分离得到,化合物7为首次从该植物中分离得到,化合物2,7,10,11具有抑制破骨细胞活性。
OBJECTIVE: To study the chemical constituents of rhizomes of Cimicifuga foetida and the inhibitory activity of the resulting compounds on osteoclasts. Methods: The chemical constituents were isolated and purified by chromatography, such as normal and reverse phase silica gel. The structures of compounds were identified based on the spectral data and physical and chemical properties. And carried out the study of inhibiting osteoclast activity. Results: Triterpenoids from 13 petroleum ether and ethyl acetate extracts of rhizome cortex were separated and identified as 25-O-acetyl-Cimilol (1), Cimicol ( (3) 26-deoxyacotin (4), acteoside A (5), 23-epi-26-dehydroxycotin (6) (9), (23R, 24S) 25-O-acetyl-Cortisol-3-O-β-D-pyran (23), (23R, 24S) Cortisol-3-O-β-D-xylopyranoside (11) . CONCLUSION: Compound 5 was isolated from this genus for the first time. Compound 7 was isolated from this genus for the first time. Compounds 2, 7, 10 and 11 inhibited the activity of osteoclasts.