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目的探讨血液分析仪自动检测后血细胞涂片显微镜复检的必要性。方法回顾性分析血细胞分析仪检测后手工血涂片显微镜复检的1401份标本,计算镜检符合率和不符合率。结果标本复检原因包括:无白细胞(WBC)分类结果、WBC计数异常、WBC分类异常、红细胞(RBC)或血红蛋白(HGB)异常、血小板(PLT)异常及其他。1401例复检样本中,镜检符合率为68.1%(954/1401),不符合率为31.9%(447/1401)。1401例复检标本镜检出323例异常细胞,其中原始细胞38例、幼稚细胞72例、中晚幼粒细胞13例、中性粒细胞毒性变124例、异型淋巴细胞11例、晚幼红细胞10例、红细胞形态异常26例、大血小板8例、血小板聚集15例及其他异常6例。结论血液分析仪检测后进行手工显微镜复检对于保证血细胞检验报告的准确性是十分必要的,并且能使临床减少误诊和漏诊。
Objective To investigate the necessity of blood cell smear microscopy after automatic detection of blood analyzer. Methods Retrospective analysis of 1401 samples from the manual blood smear microscopy after hematology analyzer was used to calculate the coincidence rate and non-coincidence rate of microscopic examination. Reasons for review of the results include: no white blood cell (WBC) classification results, WBC count abnormalities, WBC classification abnormalities, red blood cell (RBC) or hemoglobin (HGB) abnormalities, platelet abnormalities (PLT) and others. Of the 1401 retest samples, the coincidence rate of microscopic examination was 68.1% (954/1401) and the non - coincidence rate was 31.9% (447/1401). There were 323 abnormal cells in 1401 re-examination specimens, of which 38 were primitive cells, 72 naive cells, 13 middle and late promyelocytes, 124 neutrophils, 11 allogenic lymphocytes, 10 cases, abnormal erythrocyte morphology in 26 cases, 8 cases of large platelet, platelet aggregation in 15 cases and other abnormalities in 6 cases. Conclusion The hand-held microscopic examination after blood analyzer test is very necessary to ensure the accuracy of the blood test report, and can reduce the clinical misdiagnosis and missed diagnosis.