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目的:建立乳康胶囊的质量控制方法。方法:对乳康胶囊中青皮、柴胡进行薄层色谱鉴别;采用HPLC对柴胡皂苷a、柴胡皂苷d进行总含量测定。结果:薄层色谱斑点清晰,阴性对照无干扰;柴胡皂苷a进样量在2.04～14.28μg(r=0.999 8)、柴胡皂苷d进样量在2.52-17.64μg(r=0.9995)线性关系良好。平均回收率为99.15%,RSD 0.54%(n=9)。结论:建立的TLC和HPLC专属性强、准确度高、重复性好,可用于乳康胶囊的质量控制。
Objective: To establish the quality control method of Rukang capsule. Methods: The TLC and RP-HPLC were used to determine the total content of saikosaponin a and saikosaponin d in Rukang Capsules. Results: The TLC spots were clear and no interference was observed in the negative control. The injection volume of saikosaponin a was 2.04-14.28μg (r = 0.999 8), and the saikosaponin d injection volume was 2.52-17.64μg (r = 0.9995) Good relationship. The average recovery was 99.15%, RSD 0.54% (n = 9). Conclusion: TLC and HPLC are highly specific, accurate and repeatable, and can be used for the quality control of Rukang capsule.