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作者证实未免疫的小鼠脾细胞与用实验性心肌局部缺血的狗血清处理过的SRBC所形成的花瓣显著增加。本文介绍在心肌梗塞患者血清影响下花瓣形成增强现象的临床意义。方法大致如下:取心肌梗塞患者的血清置56℃30分钟(灭活补体),以后与SRBC混合,在37℃孵育3小时,接着用冷的等渗氯化钠溶液洗二次,再悬浮于199培养液中。取未免疫的Wistar大鼠脾细胞悬液0.5毫升(2×10~7有核细胞/毫升)加等量的SRBC悬液(5×10~7/毫升),分别于37℃孵育5分钟和1小时15分钟,最后在小室计算花瓣形成细胞数,并以10~6有核细胞中花瓣形成细胞数表示之。前者为早期或活性
The authors demonstrated a significant increase in the petals formed by unimmunized mouse splenocytes and SRBC treated with experimental myocardial ischemic dog serum. This article describes the clinical significance of enhanced petal formation under the influence of serum in patients with myocardial infarction. The procedure is as follows: Serum from patients with myocardial infarction is placed in a 56 ° C, 30 min, inactivated complement, mixed with SRBC, incubated at 37 ° C for 3 hours, washed twice with cold isotonic sodium chloride solution and resuspended in 199 medium. 0.5 ml (2 × 10 -7 nucleated cells / ml) of unimmunized Wistar rat spleen cell suspension was added an equal volume of SRBC suspension (5 × 10 -7 / ml) and incubated at 37 ° C. for 5 minutes and 1 hour and 15 minutes, and finally calculate the number of petal-forming cells in the cell and express it as the number of petal-forming cells in 10 to 6 nucleated cells. The former is early or active