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近年来,报道IgE与肿瘤相关,为了解食管癌患者血清IgE含量变化。我们对34例食管癌患者进行了血清IgE检测,并就临床价值作初步探讨。 对象和方法 一、对象:正常人组35例(男30,女5),年龄30～62岁,均为我院门诊体检合格的健康人,肝、肾功能试验正常。病人组34例(男30,女4),年龄32～65岁,均为本院住院患者,全部病例均经临床明确诊断(包括X线、病理切片证实)。患者于手术前一周和术后三周分别抽取空腹静脉血,分离血清,-20℃保存待测,一周内测定完毕。 二、方法:采用IRMA(免疫放射法),IgE试剂盒由天津DPC公司提供,操作按说明书。
In recent years, IgE has been reported to be associated with tumors in order to understand changes in serum IgE levels in esophageal cancer patients. We performed serum IgE detection in 34 patients with esophageal cancer and discussed the clinical value. Subjects and Methods I. Object: 35 normal subjects (30 males and 5 females) aged 30-62 years old were healthy people who passed the physical examination in our hospital. The liver and kidney function tests were normal. The patient group consisted of 34 patients (30 males and 4 females) aged 32-65 years old. All patients were hospitalized in our hospital. All cases were confirmed by clinical diagnosis (including X-ray and pathological sections). Patients were collected fasting venous blood one week before surgery and three weeks after surgery. Serum was separated and stored at -20°C. The determination was completed within one week. Second, the method: using IRMA (immuno-irradiation method), IgE kit provided by the Tianjin DPC company, operating instructions.