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作者使用Fura-2作为Ca~(++)指示剂,观察了促甲状腺激素释放激素(TRH)和白细胞介素-1β(IL—1β)对体外培养的大鼠GH_3垂体细胞内Ca~(++)([Ca~(++)]i)水平的影响。结果显示10~(-7)～10~(-9)mol/L浓度TRH促进[Ca~(++)]i动员,其程度呈TRH剂量依赖性。单用0.01～10U/ml IL—1β未增加工[Ca~(++)]i水平。用0.1U/ml IL—1β与待测细胞温育1和3分钟后,加入TRH,[Ca~(++)]i释放则受明显抑制;而TRH与IL—1β同时使用,抑制作用则明显减弱。本研究证实[Ca~(++)]i对TRH在垂体细胞内的作用起重要信息传递作用,一定剂量IL—1β可能通过抑制TRH诱导的[Ca~(++)]i释放影响垂体功能。
The purpose of this study was to investigate the effects of thyroid-stimulating hormone releasing hormone (TRH) and interleukin-1β (IL-1β) on the expression of Ca ~ (+) in rat pituitary GH_3 cells using Fura- +) ([Ca ~ (++)] i) level. The results showed that 10 ~ (-7) ~ 10 ~ (-9) mol / L concentration of TRH promoted [Ca ~ (++)] i mobilization in a dose - dependent manner. Single use of 0.01 ~ 10U / ml IL-1β did not increase [Ca ~ (++)] i levels. After the cells were incubated with 0.1 U / ml IL-1β for 1 and 3 minutes, the release of [Ca ~ (++)] i was significantly inhibited by the addition of TRH, while TRH was used simultaneously with IL-1β, while the inhibitory effect was Obviously weakened. This study demonstrates that [Ca ~ (++)] i plays an important role in the transmission of TRH in pituitary cells. A certain dose of IL-1β may affect pituitary function by inhibiting TRH-induced [Ca ~ (++)] i release .