为建立一个从病理组织诊断吗啡中毒的方法，研究吗啡在体内的分布，应用盐酸吗啡针剂对家兔进行慢性染毒，采用免疫荧光技术，原位观察吗啡在染毒家兔肝、脾、肾及脑组织中的分布及各脏器中吗啡含量的差异，同时采用 HPLC检测同一取材组织中的吗啡含量。通过对比分析这两项指标，认为吗啡免疫荧光组织化学技术可以作为一个简便、快速、较特异地鉴定吗啡类中毒的方法。 To establish a method to diagnose morphine poisoning from pathological tissues, the distribution of morphine in vivo was studied. Rabbits were treated with morphine hydrochloride by chronic injection. Immunofluorescence technique was used to observe morphine in liver, spleen and kidney And the distribution of brain tissue and morphine contents in different organs. Meanwhile, the content of morphine in the same tissue was detected by HPLC. By comparative analysis of these two indicators, morphine immunofluorescence histochemistry technology can be used as a simple, rapid and specific method to identify morphine poisoning.