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本研究采用1-苯基-3-甲基-5-吡唑啉酮(PMP)作为还原醛糖的标记识别分子,利用高效液相色谱-紫外检测器(HPLC-UV)和普通C18色谱柱,建立了10种常见单糖在13.5min内的快速HPLC-UV分析鉴别技术,并成功地用于植物药水飞蓟多糖中单糖的组成分析。结果表明,醛糖的PMP标记与流动相三乙胺(TEA)配比分离调节相结合,可大幅度调节系列单糖PMP标记物的分离度,具有很高的分离可控性;水飞蓟多糖由甘露糖、鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、木糖、半乳糖及阿拉伯糖8种单糖组成,其摩尔比为0.66∶0.84∶0.58∶1.0∶1.6∶0.69∶2.7∶4.8;样品测定回收率为92.4%～104.0%。该方法简单、快速、准确,可用于水飞蓟多糖的有效质量控制。
In this study, 1-phenyl-3-methyl-5-pyrazolone (PMP) was used as a molecular marker to reduce aldose. HPLC- , Established rapid HPLC-UV analysis of ten common monosaccharides within 13.5 minutes, and successfully used in the composition analysis of the monosaccharide in the plant thistle polysaccharides. The results showed that the combination of the PMP labeling of aldose and the separation and adjustment of the mobile phase triethylamine (TEA) ratio could greatly adjust the separation of the series of monosaccharide PMP markers with high separation and controllability. The polysaccharides consisted of 8 monosaccharides of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose and arabinose in a molar ratio of 0.66: 0.84: 0.58: 1.0: 1.6: 2.7: 4.8; the recovery of the sample was 92.4% ~ 104.0%. The method is simple, rapid and accurate and can be used for effective quality control of Silybum marianum polysaccharide.