以水稻核基因型为珍汕97的4种同核异质雄性不育细胞质(D珍汕97A、G珍汕97A、T珍汕97A、W珍汕97A)及其保持系正常细胞质(珍汕97B)为对象,取其一叶一心期幼苗为试材,用对野败型雄性不育细胞质具有专化侵染性的稻瘟病茵90-2菌株产生的粗毒素进行浸根处理8h后,用等电聚焦电泳法测定叶片中酯酶同工酶谱的变化情况。结果表明,同核情况下不同细胞质试材的酯酶同工酶谱存在不同程度的差异,毒素处理后它们酯酶同工酶谱的反应也存在不同程度的区别。 Four isoplasmic male sterility cytoplasms (D Zhenshan 97A, G Zhenshan 97A, T Zhenshan 97A, W Zhenshan 97A) and their maintainer line cytoplasm (Zhenshan 97) 97B) were taken as experimental materials. The crude toxin produced by the strain 90-3 of wild-type male sterile cytoplasm of Blastomyces blastema was used for root immersion for 8 hours. Isoelectric focusing electrophoresis was used to determine the change of esterase isoenzyme profile in leaves. The results showed that esterase isoenzymes of different cytoplasm tested materials differed to some extent in the same nuclear condition, and there were some differences in their esterase isozymes after the toxin treatment.