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目的观察研究特异性免疫治疗对BALB/c小鼠特应性皮炎模型的疗效。方法雌性的SPF级纯系BALB/c小鼠36只,随机分为空白对照组、卵清蛋白激发组、丙酮激发组三组,每组12只。应用尘螨溶液诱导小鼠形成过敏体质,再分别用0.5%的丙酮和卵清蛋白诱导小鼠产生特应性皮炎,对照组选择等量PBS致敏处理。造模成功后,每组随机选择6只小鼠取血,酶联免疫吸附法(Elisa)检测血清总IgE水平变化。取血完毕后取相应小鼠皮肤进行病理切片。剩余小鼠应用阿罗格脱敏试剂进行特异性免疫治疗(specific immunotherapy,SIT)1周,对照组未予任何处理,取血检测血清IgE水平,相应皮损进行病理组织切片。结果接触卵清蛋白及丙酮的小鼠皮肤局部呈现表皮增生、细胞浸润等炎症性改变,与正常对照组相比较,临床表现及病理符合特应性皮炎改变;实验组小鼠血清总IgE(卵清组与丙酮组数值:3 479.899±758.945pg/mL、3 809.807±532.886pg/mL)与对照组数值(1 148.970±131.648pg/mL)相比明显升高,差异具有统计学意义(P<0.05)。经特异性免疫治疗一周后,实验组小鼠血清总IgE水平与前对照降低,表皮增厚及炎性渗出症状改善。结论卵清蛋白与丙酮在尘螨致敏小鼠表皮上均可产生类似人类特应性皮炎的临床及病理改变,为AD发病机制及治疗的研究提供一个较理想的动物模型。特异性免疫治疗对人类特应性皮炎炎症的转归有一定效果。
Objective To investigate the effect of specific immunotherapy on atopic dermatitis in BALB / c mice. Methods Thirty-six female SPF pure BALB / c mice were randomly divided into blank control group, ovalbumin challenge group and acetone challenge group, with 12 mice in each group. Dust mite solution was used to induce the formation of allergic mice. Atopic dermatitis was induced in mice by 0.5% acetone and ovalbumin respectively. The control group was sensitized with equal volume of PBS. After successful modeling, each group of 6 mice were randomly selected to take blood, Elisa serum total IgE levels were measured. After taking the blood, take the corresponding mouse skin pathology. The remaining mice were treated with Arroge desensitization reagent for 1 week, and the control group received no treatment. Blood samples were taken for serum IgE level and histological sections were taken for the corresponding lesions. Results The mouse skin exposed to ovalbumin and acetone showed local inflammatory changes such as epidermal hyperplasia and cell infiltration. Compared with the normal control group, the clinical manifestations and pathological changes were consistent with those of atopic dermatitis. The serum total IgE (4789.899 ± 758.945pg / mL, 3 809.807 ± 532.886pg / mL) was significantly higher than that of the control group (1 148.970 ± 131.648pg / mL), the difference was statistically significant (P < 0.05). One week after the specific immunotherapy, the serum total IgE level in the experimental group decreased compared with the previous control, and the symptoms of epidermal thickening and inflammatory exudation were improved. Conclusions Ovalbumin and acetone can both produce clinical and pathological changes similar to human atopic dermatitis on the skin of sensitized mice and provide an ideal animal model for the pathogenesis and treatment of AD. Specific immunotherapy on the outcome of human atopic dermatitis inflammation has a certain effect.