Edman降解是蛋白质与肽顺序测定的最常用方法。降解产物经酸性转化后生成各种氨基酸的苯基乙内硫酰脲衍生物(PTH—氨基酸)。鉴定PTH—氨基酸的经典方法有硅胶薄层法;聚酰胺薄膜双向层析法,气相色谱法以及酸性与碱性回原水解法等。这些方法由于存在一些明显的缺点,在实际应用上都不甚理想。 Edman degradation is the most commonly used method of protein and peptide sequencing. The degradation products were acid converted to give the phenylglycerol thiourea derivatives (PTH-amino acids) of various amino acids. The classic method to identify PTH-amino acids is silica gel thin layer method; polyamide thin film bi-directional chromatography, gas chromatography and acidic and alkaline back-hydrolysis methods. Due to some obvious shortcomings, these methods are not ideal in practical application.