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目的 探讨原发性肝癌经肝动脉化疗栓塞(TACE)前后肝癌细胞的共刺激分子4 -1BB(CD13 7)的配体(4- 1BBL)的变化及意义。方法 选取经TACE治疗后二期手术切除的原发性肝癌患者标本14例(TACE组)和未经TACE单纯手术切除的原发性肝癌患者标本2 1例(单纯手术组) ,应用免疫组织化学方法和RT -PCR方法检测肝癌细胞的4 -1BBL及4- 1BBLmRNA表达情况。结果 TACE组肝癌细胞中4 -1BBL和4- 1BBLmRNA的表达明显低于单纯手术组。结论 TACE后肝癌细胞中4 -1BBL的表达下调会影响机体的抗肿瘤免疫反应,并可能是TACE治疗原发性肝癌难以根治并易复发、转移的原因之一。
Objective To investigate the changes of 4-1BBL ligand (4-1BBL) in hepatocellular carcinoma before and after transcatheter arterial chemoembolization (TACE). Methods TACE specimens from 14 patients with primary liver cancer (TACE group) and 21 patients with primary hepatocellular carcinoma (TACE group) without surgical removal of TACE were included in this study. Immunohistochemistry Methods The mRNA expressions of 4 -1 BBL and 4 -1 1BBL in hepatocellular carcinoma cells were detected by RT-PCR. Results The expression of 4-1BBL and 4-1BBL mRNA in TACE group was significantly lower than that in the simple operation group. Conclusions The down-regulation of 4 -1 BBL expression in hepatocellular carcinoma cells after TACE may affect the anti-tumor immune response in the body and may be one of the reasons that TACE is difficult to cure and recurrence and metastasis of primary liver cancer.