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利用分子生物学中常用的DNA分子标记对世界各地现存的野生和栽培的啤酒花种质资源遗传多样性研究的应用作一综述。通过归纳总结,发现DNA分子标记相比形态学标记和细胞学标记具有结果准确、稳定的特点,常用的分子标记技术有RAPD、RFLP、ISSR、SSR、AFLP、EST等;研究发现北美洲的啤酒花遗传多样性要高于欧洲的啤酒花,基因变异程度也相对较高;野生啤酒花的基因序列具有丰富的基因多样性,可在分子杂交遗传育种中作为一个种质去改善栽培品种的某些不良性状。因此,利用分子标记研究啤酒花的遗传多样性将对啤酒花的育种提供理论指导和技术支持,目前较为理想的技术是SSR和AFLP。
The use of DNA molecular markers commonly used in molecular biology to review the genetic diversity of wild and cultivated hops germplasm resources around the world is reviewed. Through summarizing and summarizing, it is found that DNA molecular markers are accurate and stable compared with morphological markers and cytological markers. RAPD, RFLP, ISSR, SSR, AFLP, EST and other molecular markers are commonly used in molecular marker. The results show that North American hops Genetic diversity is higher than that in Europe, and genetic variation is relatively high. The genetic sequence of wild hops is rich in genetic diversity and can be used as a germplasm in molecular hybrid genetic breeding to improve some bad traits of cultivated cultivars . Therefore, using molecular markers to study the genetic diversity of hops will provide theoretical guidance and technical support for hops breeding. At present, the ideal technologies are SSR and AFLP.