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目的了解隆安县乙型肝炎病毒(HBV)的分子流行病学特征。方法以分层抽样方法对隆安县全县12个乡、镇108个自然村30~50岁居民进行调查,抽取静脉血,用酶联免疫吸附法(ELISA)检测乙型肝炎表面抗原(HBsAg),用套式聚合酶链反应(nPCR)对HBsAg阳性者检测血清HBV DNA。结果在27379名受检者中,HBsAg阳性率为14.52%,各乡、镇的感染率不尽相同,平原片显著高于山区和丘陵片;男、女阳性率分别为16.66%和12.60%,男性明显高于女性;各年龄组HBsAg阳性率随年龄增长而呈下降趋势。在3975名HBsAg阳性者中,HBV DNA阳性率40.35%,阳性率在各乡、镇的分布也不一致;男、女HBV DNA阳性率分别为42.82%和37.44%,男性显著高于女性;也呈现随年龄增长而下降趋势。结论隆安县仍是我区乙肝病毒高流行区,应加强防治。
Objective To understand the molecular epidemiology of hepatitis B virus (HBV) in Longan County. Methods A stratified sampling method was used to investigate residents from 30 to 50 years of age in 108 natural villages in 12 townships and townships in Long’an County. Venous blood was collected for detection of hepatitis B surface antigen (HBsAg) by enzyme-linked immunosorbent assay (ELISA) Serum HBV DNA was detected in HBsAg positive patients by nested polymerase chain reaction (nPCR). Results Among 27 379 subjects, the positive rate of HBsAg was 14.52%. The infection rate in each township and town was different. The plain tablets were significantly higher than those in mountainous and hilly areas. The positive rates of male and female were 16.66% and 12.60% Men were significantly higher than women; HBsAg positive rate of all age groups showed a downward trend with age. In 3975 HBsAg positive persons, the positive rate of HBV DNA was 40.35%, and the positive rate was also inconsistent in each township and town. The positive rates of HBV DNA in male and female were 42.82% and 37.44% respectively, which were significantly higher in males than in females Decreasing trend with age. Conclusion Longan County is still a high endemic area of hepatitis B virus in our region, and should be strengthened.