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目的探讨ΔNp63 mRNA在膀胱移行细胞癌(TCCB)中的表达及意义。方法采用逆转录聚合酶链反应(RT-PCR)方法检测48例TCCB患者肿瘤组织、10例对照膀胱组织ΔNp63 mRNA的表达,并分析ΔNp63 mRNA表达与TCCB临床分期、病理分级等的关系。结果48例TCCB组织中ΔNp63 mRNA表达阳性率为75.0%(36/48),对照组膀胱组织中ΔNp63蛋白表达阳性率为0.0%(0/10),TCCB组ΔNp63 mRNA表达阳性率明显高于对照组,差异有统计学意义(P<0.05)。不同临床分期肿瘤组织中ΔNp63 mRNA表达阳性率、各组间ΔNp63 mRNA表达阳性率差异无统计学意义(P>0.05)。不同病理分级肿瘤组织中ΔNp63 mRNA表达阳性率、各组间ΔNp63mRNA表达阳性率差异无统计学意义(P>0.05)。结论TCCB组ΔNp63mRNA表达水平均明显高于对照组,提示ΔNp63与TCCB的发生密切相关,其可能为TCCB发生的重要促进因素,ΔNp63 mR-NA表达水平和TCCB的临床分期、病理分级没有相关性,故ΔNp63在TCCB进展中的作用有待进一步研究。
Objective To investigate the expression of ΔNp63 mRNA in bladder transitional cell carcinoma (TCCB) and its significance. Methods The expression of ΔNp63 mRNA in 48 cases of TCCB patients and 10 cases of control bladder tissues was detected by RT-PCR. The relationship between ΔNp63 mRNA expression and the clinical stage, pathological grade of TCCB was analyzed. Results The positive rate of ΔNp63 mRNA in 48 cases of TCCB was 75.0% (36/48), while the positive rate of ΔNp63 in control bladder was 0.0% (0/10). The positive rate of ΔNp63 mRNA in TCCB was significantly higher than that of control Group, the difference was statistically significant (P <0.05). The positive rates of ΔNp63 mRNA in different clinical staging tumor tissues showed no significant difference (P> 0.05). The positive rates of ΔNp63 mRNA in different pathological grading tumor tissues showed no significant difference (P> 0.05). Conclusions The expression of ΔNp63 mRNA in TCCB group was significantly higher than that in control group, suggesting that ΔNp63 is closely related to the occurrence of TCCB, which may be an important contributing factor for TCCB. The expression of ΔNp63 mR-NA has no correlation with clinical stage and pathological grade of TCCB, Therefore, the role of ΔNp63 in the progress of TCCB needs further study.