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目的:建立中药饮片制草乌、制川乌中次乌头碱、乌头碱的含量测定方法。方法:采用KromasilC18(5μm,4.6mm×250mm)作为色谱柱,甲醇-水-氯仿-三乙胺作为流动相,检测波长为230nm,柱温为室温。结果:乌头碱在9.63～96.26μg/mL范围内呈良好的线性关系,次乌头碱在24.2～242μg/mL范围内呈良好的线性关系,加样回收率大于96%(RSD<2%)。结论:该法操作简便,结果可靠,可用于中药饮片制川乌、制草乌中乌头碱、次乌头碱的含量测定。
Objective: To establish a method for the determination of aconitine and aconitine in Radix Aconiti Kusnezoffii Preparata. Methods: Kromasil C18 (5μm, 4.6mm × 250mm) was used as the column and methanol-water-chloroform-triethylamine was used as the mobile phase. The detection wavelength was 230nm and the column temperature was room temperature. Results: Aconitine showed a good linearity in the range of 9.63 ~ 96.26μg / mL. Hypaconitine showed a good linearity in the range of 24.2 ~ 242μg / mL. The recoveries were higher than 96% (RSD <2% ). Conclusion: The method is simple and reliable, and can be used for the determination of aconitine and hypaconitine in Radix Aconiti Preparata.