论文部分内容阅读
背景 对A型红细胞上的糖结合物α3N-乙酰半乳糖胺抗原决定族酶化使成为O型通用红细胞,为A细胞向O型通用细胞转化提供了基本的理论。为了探讨在B或O型受者中引起免疫应答的A抗原表位对酶化的敏感性,作者用体外试验,比较了A抗原对不同浓度的从Ruminococcus torques纯化的外切酶-α-N-乙酰半乳糖胺酶(A-酶)酶切的敏感性。方法和结果 在pH6.0的缓冲液中,新鲜的Al细胞和0.1-05.0IUA酶/ml压积红细胞一起孵育2小时(T)或新鲜Al细胞在缓冲液中单独孵育2小时(C)。比较C和T的免疫应答情况。在使用了0.2-0.5UA-酶/ml之后,包被了IgG抗-A单克隆抗体(mAb)BG-2的A细胞,免疫粘附到U-937单核细胞上的细胞(％玫瑰花结形成)上的平均数从28.9±4.5％(C)降到0.2±0.2％(T)。在
Background The family of α3N-acetylgalactosamine antigen-binding glycoproteins on type A erythrocytes have made O-type red cells universal, providing a basic theory for the transformation of A cells into O-type cells. In order to investigate the sensitivity of the A-epitopes that elicit an immune response in B or O-type recipients to enzymatic reactions, the authors compared in vitro experiments the effect of A-antigen on different concentrations of exonuclease-alpha-N purified from Ruminococcus torques - acetylgalactosamine (A-enzyme) digestion sensitivity. Methods and Results Fresh Al cells were incubated with 0.1-05.0 IUA enzyme / ml hematocrit for 2 h (T) or fresh Al cells incubated in buffer alone at pH 6.0 for 2 h (C). Compare the C and T immune responses. A cells coated with IgG anti-A monoclonal antibody (mAb) BG-2 after 0.2-0.5 UA-enzyme / ml was used, cells adherent to U-937 monocytes Knot formation) decreased from 28.9 ± 4.5% (C) to 0.2 ± 0.2% (T). in