以珠状的对氨基苯砜乙基交联琼脂为载体,通过重氮化反应共价连接辅酶A(Co-A)。CoA被固定化的量与载体上功能团含量密切相关,随载体上芳香胺基的增多,CoA的偶联量增加。偶联反应液的pH明显影响固定化CoA的性质。酸性pH下偶联,固定化效率显著高于碱性条件下偶联;但是 Coenzyme A (Co-A) was covalently linked by a diazotization reaction using beaded p-aminophenylsulfone ethyl-cross-linked agar as a carrier. The amount of CoA immobilized is closely related to the amount of functional groups on the support. With the increase of the number of aromatic amine groups on the support, the amount of CoA coupled increases. The pH of the coupling reaction significantly affects the properties of the immobilized CoA. Coupling at acidic pH, the immobilization efficiency is significantly higher than that under alkaline conditions; however