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为了更好地了解甲型肝炎病毒(HAV)突变程度对病毒在细胞培养上增殖能力的影响及引起病毒减毒的因素,作者比较了不同代次HAV HM-175株的5′端非编码区和2BC区的基因序列及这些病毒在几种细胞上的增殖能力和对狨猴、黑猩猩的致病力。 作者将HAV HM-175株在原代非洲绿猴肾(AGMK)细胞上分别传9或10代(p10)、21代(p21)和32代(p32),用聚合酶链反应法对病毒的5′和2BC区进行扩增,然后作序列测定。 对不同传代水平HM-175株核苷酸序列的比较结果表明,在5′端非编码区和p2区的2BC基因存在突变,野毒株和经细胞培养传35代的适应株(p35)在所有13个位点上均不同。
To better understand the effect of hepatitis A virus (HAV) mutation on the ability of virus to proliferate on cell culture and the factors that cause virus attenuation, the authors compared the 5 ’non-coding region And 2BC region of the gene sequence and the proliferation of these viruses on several cells and the ability to marmoset, chimpanzee virulence. The HAV strain HM-175 was passaged on the primary African green monkey kidney (AGMK) cells for passage 9 or 10 (p10), passage 21 (p21) and passage 32 (p32) ’And 2BC area amplification, and then for sequencing. Comparison of the nucleotide sequences of HM-175 strains at different passage levels showed that there were mutations in the 2BC gene at the non-coding region and the p2 region at the 5 ’end. The wild type strain and the adapted strain 35 (p35) All 13 sites are different.