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目的从行为学角度进一步探讨逍遥散治疗肝郁脾虚证的调节机制。方法 75只SD雄性大鼠随机分为5组,正常组、模型组、假手术组、α-氨基羟甲基恶唑丙酸(AMPA)组和逍遥散组。以21 d慢性束缚应激方法造肝郁脾虚证模型,于模型大鼠双侧海马CA1区,埋管微量注射AMPA受体的激动剂AMPA,逍遥散组造模方法和AMPA组尽可能相似,突出逍遥散和AMPA 2种干预的可比性。于第1、7、14、21 d分别比较AMPA组和逍遥散组行为变化各项指标趋势是否一致。结果模型组大鼠逐步呈现肝郁脾虚证表现,AMPA组、逍遥散组大鼠焦躁状态逐步得到抑制。造模第21天,与正常组比较,AMPA组和逍遥散组大鼠穿格次数、站立次数、修饰次数等指标均无统计学意义(P>0.05)。结论从宏观行为学角度,初步推断逍遥散可能通过纠正杏仁核和海马的“兴奋-抑制”失衡,重建稳态,来治疗肝郁脾虚证。
Objective To further explore the regulatory mechanism of Xiaoyaosan in treating liver-qi stagnation and spleen-deficiency syndrome from the perspective of behavior. Methods Seventy-five SD male rats were randomly divided into 5 groups: normal group, model group, sham operation group, AMPA group and Xiaoyao powder group. The model of liver-qi stagnation and spleen-asthenia syndrome was induced by chronic restraint stress for 21 days. AMPA and AMPA-stimulating agents AMPA and AMPA were injected into bilateral hippocampal CA1 region of model rats as similar as AMPA group. Highlight Xiaoyaosan and AMPA 2 kinds of interventions comparability. On the 1st, 7th, 14th and 21st days, we compared the trends of AMPA group and Xiaoyao powder group respectively. Results The rats in model group showed the manifestation of stagnation of liver-qi and spleen-asthenic gradually. The anxious state of rats in AMPA group and Xiaoyao powder group was gradually suppressed. On the 21st day of modeling, compared with the normal group, there were no significant differences in the number of wearing, the number of standing, the number of modification and so on in AMPA group and Xiaoyaosan group (P> 0.05). Conclusions From the perspective of macroscopic behavior, Xiaoyaosan may be preliminarily concluded that Xiaoyaosan may treat stagnation of liver-qi and spleen by correcting the “excitement-inhibition” imbalance of amygdala and hippocampus and rebuilding the homeostasis.