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目的本研究通过探讨胃癌组织中CD34和Ki67蛋白表达与临床病理特征的相关性,进而分析其临床意义。方法收集在新疆医科大学第一附属医院手术切除及胃镜活检病理明确诊断为胃癌的组织标本,采用Eli Vision TMplus免疫组织化学染色方法检测,其中临床资料完整的245例胃腺癌标本中CD34和Ki67蛋白表达。结果 245例胃癌组织中CD34和Ki67蛋白的阳性表达率分别为61.2%和95.1%,且呈正相关,但癌旁组织中均未见其阳性表达。CD34和Ki67蛋白表达有淋巴结转移患者显著高于无淋巴结转移患者,且与肿瘤浸润深度呈正相关,与肿瘤分化程度呈负相关;CD34表达与肿瘤大小、有无脉管神经侵犯及病理分期呈正相关。结论 D34和Ki67在胃癌组织中高表达与肿瘤细胞增殖、血管形成以及预后有关。联合检测CD34和Ki67蛋白表达有助于了解胃癌发生发展、浸润转移的机制,并可作为评估胃癌恶性生物学行为及预后的参考指标。
Objective This study was to explore the clinical significance of CD34 and Ki67 protein expression in clinic and clinicopathological features of gastric cancer. Methods Tissue specimens of gastric cancer diagnosed by surgical excision and endoscopic biopsy in the First Affiliated Hospital of Xinjiang Medical University were collected and detected by Eli Vision TMplus immunohistochemical staining. Among the 245 gastric adenocarcinoma samples with complete clinical data, CD34 and Ki67 expression. Results The positive rates of CD34 and Ki67 in 245 cases of gastric cancer tissues were 61.2% and 95.1%, respectively. There was a positive correlation between them, but no positive expression was found in paracancerous tissues. The expression of CD34 and Ki67 in patients with lymph node metastasis was significantly higher than that in patients without lymph node metastasis and was positively correlated with the depth of tumor invasion and negatively correlated with the degree of tumor differentiation.The expression of CD34 was positively correlated with the size of tumor and the presence of vascular invasion and pathological stage . Conclusion The high expression of D34 and Ki67 in gastric cancer tissue is related to tumor cell proliferation, angiogenesis and prognosis. Combined detection of CD34 and Ki67 protein expression is helpful to understand the mechanism of gastric carcinogenesis, invasion and metastasis, and can be used as a reference index to evaluate malignant biological behavior and prognosis of gastric cancer.