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本文以抗人C_(?)的羊IgG为包被抗体,以HRP-HBs抗体为指示抗体,建立了可检测激活补体类HBsAg循环免疫复合物(HBsAg/C3-CIC)的C_3捕捉法酶联免疫吸附试验。检测了236例六种类型临床诊断为乙型肝炎的病人血清标本,其阳性率分别为:无症状携带者(ASC)12.9％(4/31),急性肝炎(AH)36.7％(22/60),慢性迁延性肝炎(CPH)33.3％(7/21),慢性活动性肝炎(CAH)59.6％(34/57),重型肝炎(SH)77.8％(14/18),肝炎后肝硬化(PLC)67.3％(33/49),阳性率与肝损严重程度明显相关(P<0.01)。认为HBs-Ag/C3-CIC可能在乙型肝炎病毒引起的慢性活动性肝炎、重型肝炎和肝炎后肝硬化的发病过程中起重要作用,并可作为乙型肝炎的诊断、临床分型和预后判断的指标之一。
In this paper, anti-human C_ (?) Goat IgG coated antibody, HRP-HBs antibody as an indicator antibody, the establishment of a detectable activation of complement HBsAg circulating immune complexes (HBsAg / C3-CIC) C_3 capture enzyme Immunoassay test. Serum samples from 236 patients with six types of clinically diagnosed hepatitis B were detected. The positive rates were 12.9% (4/31) in asymptomatic carriers (ASC) and 36.7% (AH) in 22.7% of patients with acute hepatitis (AH) , 33.3% (7/21) of chronic persistent hepatitis (CPH), 59.6% (34/57) of chronic active hepatitis (CA), 77.8% (14/18) of severe hepatitis (SH) PLC) 67.3% (33/49), the positive rate was significantly related to the severity of liver damage (P <0.01). It is considered that HBs-Ag / C3-CIC may play an important role in the pathogenesis of chronic active hepatitis caused by Hepatitis B virus, severe hepatitis and posthepatitis cirrhosis, and can be used as diagnosis, clinical classification and prognosis of hepatitis B One of the indicators to judge.