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目的鉴定并分析类孟买型血清学特点,探讨输血策略。方法采用ABO血型正反定型、H抗原检测及红细胞吸收放散试验,采用PCR-SSP法进行FUT1基因测序,采用配合型输血方案进行类孟买型输血策略研究。结果 6例被检者均确认为类孟买型,分别为Bm~h4例、Am~h2例;FUT1基因测序547-552del AG 4例、682 AG 2例;例1、例2与10份B型悬浮红细胞、10份O型洗涤红细胞进行交叉配血试验,结果显示在室温有2份交叉配血不合,在37℃交叉配血全部相合。结论罕见类孟买型血清学鉴定研究,对后续患者的输血策略具有重要意义。
Objective To identify and analyze serotypes of Mumbai type and discuss the strategy of blood transfusion. Methods The ABO blood type positive and negative typing, H antigen test and erythrocyte absorption and dissipation test were used. FUT1 gene sequencing was carried out by PCR-SSP method. RESULTS: All the 6 subjects were classified as Mumbai, including 4 cases of Bm-h and 2 cases of Am-h, 4 cases of FUT1 gene sequencing 547-552del AG and 2 cases of 682 AG. Cases 1, 2 and 10 cases of type B Resuspended erythrocytes, 10 copies of O-washed red blood cells cross-matching test showed that at room temperature with two cross-mismatched blood, cross-matching at 37 ℃ all match. Conclusion The rare Mumbai type serological identification study is of great significance to the follow-up of patients with blood transfusion strategies.